High-Efficiency Gene Transfer and Selection of Human Hematopoietic Progenitor Cells with a Hybrid EBVlRetroviral Vector Expressing the Green Fluorescence Protein1
نویسندگان
چکیده
Department of Experimental Oncology, European Institute of Oncology, 20141 Milan, Italy [P. G. P., L Li; Istituto di Medicine Interna e Scienze Oncologiche, Perugia University, 06100 Perugia, Italy (Fr. G., A. M., D. R., Fa. G., P. G. P.]; Hematology Oncology Laboratory. Istituto Superiore di Sanità 00161 Rome, Italy (Fr. G., M. V., C. P.]; Department of Molecular Pharmacology, Stanford University, Stanford, California 94305 fT. K., G. P. N.]; and Thomas Jefferson University, Kimmel Cancer Center, Philadelphia, Pennsylvania 19107 (C. P.]
منابع مشابه
High-efficiency gene transfer and selection of human hematopoietic progenitor cells with a hybrid EBV/retroviral vector expressing the green fluorescence protein.
We report a retroviral expression vector (PINCO) that allows high-efficiency gene transfer and selection of hemopoietic progenitor cells (HPCs). The main characteristics of this vector are the presence outside the two long terminal repeats of the EBV origin of replication and the EBNA-1 gene and the presence in the retrovirus of the cDNA that encodes for the enhanced green fluorescence protein ...
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